MCQ Microbiology Answer 37
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The answer is: B
DNA polymerase isolated from the hot springs thermophilic bacterium named Thermus aquaticus is essential for the polymerase chain reaction (PCR) process because of its stability at high temperatures (95°C). While the E. coli enzyme can be used, the enzyme itself becomes denatured, fewer cycles are possible, and nonspecific reactions occur because of hybridization of primers to nontarget DNA. The use of Taq polymerase allows DNA copying at 72°C rather than 37°C, which further reduces nonspecific hybridization.
Tags: MCQ, Microbiology, Polymerase, PCR
Category: Microbiology MCQs
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